The usual suspect: solving the case of RNase contamination in RNA analysis

RNase contamination can cause chaos in RNA analysis labs. What are these problems and how can they be solved?

RNase is the enzyme responsible for RNA degradation in living organisms. They play a key role in the maturation of RNA molecules and are a first line of defense against RNA-containing viruses, as well as in the break-down of old RNA. There are many different types of RNase, with the main type utilized in labs being RNase A, which specifically targets single-stranded RNA.

RNase A is commonly described as one of the hardiest enzymes in lab usage. RNases are generally very rich in disulfide bonds, which make them incredibly stable enzymes and can even survive autoclaving.